Table 2

Intramyocardial DNA binding activity of NF-κB and synthesis of inflammatory mediators before, during, and after CPB in pigs operated on under moderate hypothermia or normothermia

Parameter

Before CPB

Before aortic cross clamping

Before removal of aortic clamp

6 hours after CPB

28°C

37°C

28°C

37°C

28°C

37°C

28°C

37°C

Activity of NF-κB (count/mm²)

400.8 ± 14.3

439.2 ± 63.3

696.4 ± 25.9

555.1 ± 32.1

643.5 ± 7.45

507.3 ± 9.22

676.3 ± 12.8

611.0 ± 98.7

Phospho-IκB-α

0.18 ± 0.32

0.24 ± 0.45

0.71 ± 1.13

0.70 ± 0.87

0.72 ± 0.96

0.64 ± 0.49

0.85 ± 0.59

0.66 ± 0.43

iNOS

0.00 ± 0.00

0.00 ± 0.00

0.47 ± 0.54

0.29 ± 0.55

0.58 ± 0.97

0.48 ± 0.40

0.88 ± 0.58

0.47 ± 0.48

COX-2

0.09 ± 0.1

0.11 ± 0.08

0.16 ± 0.09

0.50 ± 0.18*

0.54 ± 0.06

0.68 ± 0.33

0.25 ± 0.26

0.47 ± 0.13

Results (mean ± standard deviation) are shown as the ratio of protein levels of phospho-IκB-α, iNOS and COX-2 to actin. *P < 0.05 between groups. COX, cyclooxygenase-2; CPB, cardiopulmonary bypass; iNOS, inducible nitric oxide synthase; NF-κB, nuclear factor-κB; phospho-IκB-α, phosphorylation of inhibitor of NF-κB α.

Qing et al. Critical Care 2006 10:R57   doi:10.1186/cc4886