Figure 7.

Liver histopathology. Representative samples from liver tissue sections stained with H&E, from mice treated with intraperitoneal PBS followed 14 hours later by 6 hours of spontaneous breathing (a) or mechanical ventilation (b), and from mice treated with intraperitoneal lipopolysaccharide (LPS; 1 μg/kg) followed 14 hours later by either spontaneous breathing (c) or 6 hours of mechanical ventilation (d). Livers from mice in the PBS group showed normal liver architecture, and cytoplasmic accumulation of glycogen (a, inset). Livers from mice in the mechanical ventilation (MV) group also showed normal architecture and glycogen depletion (b). Mice from the LPS group had accumulation of microvesicles in the cytoplasm (inset), which predominated in the periportal area (c). This microvesicular injury (inset) was also present in the LPS + MV group, but was markedly more extensive (d).