Rats surviving after high tidal volume ventilation show marked and reversible pulmonary and systemic changes

O Peñuelas, N Nin, M De Paula, P Fernandez-Segoviano, J Lorente and A Esteban

Hospital Universitario de Getafe, Madrid, Spain

from 26th International Symposium on Intensive Care and Emergency Medicine
Brussels, Belgium. 21–24 March 2006

Critical Care 2006, 10(Suppl 1):P6doi:10.1186/cc4353

The electronic version of this abstract is the complete one and can be found online at: http://ccforum.com/supplements/10/S1

Published:

21 March 2006

Rationale

High tidal volume (VT) ventilation (HTVV) induces pulmonary inflammation. The time course of pulmonary and systemic HTVV-induced vascular dysfunction is unknown. We tested whether rats receiving HTVV survive the insult, and we describe the time-course of the HTVV-induced vascular changes.

Methods

Normal anesthetized Sprague-Dawley rats were tracheostomized and ventilated for 1 hour with either VT = 9 ml/kg + PEEP 5 cmH₂O, or VT = 35 ml/kg + ZEEP. After the HTVV period, the tracheostomy was closed, and rats were sent back to their cages breathing room air. Other rats were sacrificed at this point in time (t = 1 hour). Rats surviving the acute period of HTVV were again intubated, monitored and then sacrificed at different points in time (24 hours, 72 hours, 168 hours). We measured the mean arterial pressure, aortic blood flow (QAo), arterial blood gases, and total protein, AST, ALT, IL-6, and VEGF serum and BAL fluid concentrations. Aortic segments and pulmonary micro vessels were mounted in myographs, and responses to acetylcholine in norepinehrine-precontracted rings were tested. Histological lung changes were studied.

Results

All lungs showed diffuse alveolar damage after HTVV at 1 and 24 hours, but histology was completely normal at t = 72 hours. HTVV induced hypotension, decreased QAo, hypoxemia, increased protein, AST, ALT, IL-6, and VEGF BAL fluid/serum concentration ratio. Acetylcholine and norephineprine-induced responses were impaired after HTVV in aortic rings. Moreover acetylcholine-induced responses in pulmonary microvessels were impaired. All these biochemical and vascular function changes normalized at t = 168 hours.

Conclusion

About half the rats receiving for a short period of time ventilation using very high VT survive. HTVV induces in a reversible fashion pulmonary and systemic inflammation and vascular dysfunction.

Acknowledgements

Funded by FIS CO3/11, GO3/063.

This article is part of the supplement: 26th International Symposium on Intensive Care and Emergency Medicine