Table 1

Real-time polymerase chain reaction primers and probes

Gene name [Genbank accession number]

Sequence

ocD2 [GenBank:EF370408]

Forward:

5'-GGACTCCGCTGTGTCTGGAA-3'

Probe:

5'-CTTGACGCCTACAAACA-3'

Reverse:

5'-GGCATCCTCGCCCAATTT-3'

ocMCT8 [GenBank:EF420874]

Forward:

5'-CCATGTGGCCTTCTACTTTGC-3'

Probe:

5'-CCCCCCATCATTGGAGCTGTCATC-3'

Reverse:

5'-TGCATCAGAGGGACGAAGAAA-3'

ocMCT10 [Genbank:EF489851]

Forward:

5'-TCCGAGCAGAAATCCAATCG-3'

Probe:

5'-TTGGGACACATCCCGGGCACC-3'

Reverse:

5'-GGCTCCCATTGCCTTTGAG-3'

ocOATP1C1 [GenBank:EF420875]

Forward:

5'-GATCAGCGGTCTTTGGTTACCT-3'

Probe:

5'-CTGTTCCTTTCCCTGTTTGCACTGGG-3'

Reverse:

5'-TGCCACATCCAAGTTTTCACA-3'

ocTRα1 [GenBank:EU489476]

Forward:

5'-GAGTGCCCCACCGAACTCT-3'

Probe:

5'-TCCCCCACTCTTCCTCGAGGTCTTTG-3'

Reverse:

5'-CCGCCTGAGGCTTTTAGACTT-3'

ocTRα2 [GenBank:EU489477]

Forward:

5'-AAGTGCAGAGTTCGATTCTGTACAA-3'

Probe:

5'-CGGGTCACTGGGCGTCCACC-3'

Reverse:

5'-GAACAACATGCATTCCGAGAAG-3'

ocTRβ1 [GenBank:EU489478]

Forward:

5'-GCGCAGCGCGTTGAA-3'

Probe:

5'-AACGAACAGTCATCACCACATCTCATCCAG-3'

Reverse:

5'-GGATGGAGCTCGTCCAAGTG-3'

ocTRβ2 [GenBank:EU489479]

Forward:

5'-GCCATCCTGACTATTTCACTGAAGA-3'

Probe:

5'-AAGCCTACTTTTTCTCAAGGGCAGTCACCG-3'

Reverse:

5'-GGGATGTACCCTTTTTTCTGAGAGT-3'

ocHPRT [GenBank:AF020294]

Forward:

5'-TGTAGATTTTATCAGACTGAAGAGCTACTGT-3'

Probe:

5'-TTTCCAGTTAAGGTTGAGAGATCATCTCCACCGAT-3'

Reverse:

5'-AAGGAAAGCAAGGTCTGCATTGTT-3'

Mebis et al. Critical Care 2009 13:R147   doi:10.1186/cc8043