Table 1

In vitro dynamic test of cytokine adsorption by Amberchrom CG161 M resin

Cytokine

Concentration (pg/ml)

Amount bound (% leakage)

(pg/ml adsorbent)

Theoretical binding capacity

(pg/ml adsorbent)

TNF-α

675 ± 72

4999 (5%) ± 1703

200,980 ± 53,607

IL-1-β

331 ± 1

61,439 (5%) ± 10,459

116,842 ± 24,707

IL-6

334 ± 59

35,729 (5%) ± 8149

912,776 ± 200,096

IL-8

340 ± 186

322 (12%) ± 161

48,940 ± 26,089

IL-10

464 ± 143

69,187 (5%) ± 31,112

972,880 ± 61,006

The conditions used for the dynamic studies on Amberchrom CG161 M resin are extensively described in the Materials and Methods. Briefly, the resin columns were perfused at a low linear velocity (0.30 ml/min). The theoretical binding capacity of the resins for individual cytokines which was calculated by extrapolation of the increase in cytokine concentration at the column outlet over time only gives a rough estimation of the actual maximal binding capacity.

Cantaluppi et al. Critical Care 2010 14:R4   doi:10.1186/cc8835