Representative photomicrographs of lung stained with H&E (left panels) and TUNEL (right panels). Animals were randomly assigned to hypovolemia (HYPO), normovolemia (NORMO) or hypervolemia (HYPER) with recruitment maneuver (RM-CPAP) or not (NR). Note that in the HYPER group, the number of apoptotic lung epithelial cells was higher than in NORMO and HYPO (arrows). Photographs were taken at an original magnification of × 200.
Silva et al. Critical Care 2010 14:R114 doi:10.1186/cc9063