A method for detection and quantification of hydroxyethyl starch in plasma
1 Clinic for Anesthesiology and Intensive Care, Jena University Hospital, Erlanger Allee 101, 07747 Jena, Germany
2 Clinic for Internal Medicine (KIM III) - Nephrology, 07747 Jena University Hospital, Jena, Germany
3 Center for Sepsis Control and Care (CSCC), Jena University Hospital, 07747 Jena, Germany
4 Max-Planck-Institut fuer Biogeochemie, Hans-Knöll-Str. 10, 07745 Jena, Germany
Critical Care 2012, 16:426 doi:10.1186/cc11302Published: 8 May 2012
First paragraph (this article has no abstract)
The use of hydroxyethylstarch (HES) is a controversial issue due to increasing evidence that HES accumulates in plasma and various tissues and therefore leads to unfavourable outcome in critically ill patients [1,2]. No simple methods are available for monitoring HES plasma levels; present technologies to measure HES are based on gas chromatography-mass spectrometry, which are time-consuming and need advanced equipment . Here, we applied Lugol's iodine solution (LUGOL; Sigma-Aldrich, Steinheim, Germany) to determinate HES concentrations in plasma and compare the results with those obtained by high-performance liquid chromatography (HPLC).