Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid `real-time' polymerase chain reaction

doi:10.1186/cc702

Critical Care
Volume 4
Issue 4

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Pirnay JP
De Vos D
Duinslaeger L
Reper P
Vandenvelde C
Cornelis P
Vanderkelen A

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De Vos D
Duinslaeger L
Reper P
Vandenvelde C
Cornelis P
Vanderkelen A
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Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid `real-time' polymerase chain reaction

Jean-Paul Pirnay¹^,2 , Daniel De Vos²^,3, Luc Duinslaeger¹, Pascal Reper¹, Christian Vandenvelde¹, Pierre Cornelis² and Alain Vanderkelen¹

¹Queen Astrid Military Hospital, Neder-Over-Heembeek, Belgium

²Flanders Interuniversity Institute of Biotechnology, Sint-Genesius-Rode, Belgium

³Innogenetics, Neder-Over-Heembeek, Belgium

author email

Critical Care 2000, 4:255-262doi:10.1186/cc702


Published:	7 July 2000

Abstract

We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 10³ colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection to result was less than 1h. RTD-PCR has potential for rapid quantitative detection of pathogens in critical care patients, enabling early and individualized treatment.