Figure 2.

Effect of thrombin on monolayer and cytoskeletal rearrangement of human primary endothelial cells derived from three different vascular beds. At early passages, cultured cells were plated in 8-well fibronectin-coated CultureSlides (Becton Dickinson, Bedford, MA, USA), 35,000 cells/well. After 24–48 hours, confluent monolayer cells were stimulated with 320 pM human thrombin (Sigma, St Louis, MO, USA) for 30 min at 37°C. Cells were fixed with 4% formaldehyde and stained for f-actin using Fluorescein isothiocyanate-conjugated phalloidin (Sigma, catalog number P5282). HCAEC, human coronary arterial endothelial cells; HMVEC-L, human lung microvascular endothelial cells; HUVEC, human umbilical venous endothelial cells. All cells were obtained from Cambrex (Walkersville, MD, USA). All images are shown at ×40 magnification.