cc145 CCJ Meeting abstract <p>Granulocyte colony-stimulating factor (G-CSF) enhances superoxide production in acute liver failure (ALF): an <it>in vivo</it> effect</p> Clapperton M Wendon J Rolando N

Institute of Liver Studies, King's College School of Medicine and Dentistry, London, UK

 Critical Care <p>18th International Symposium on Intensive Care and Emergency Medicine</p> Meeting abstracts <p>18th International Symposium on Intensive Care and Emergency Medicine</p> Brussels, Belgium 17–20 March 1998 1364-8535 1998 2 Suppl 1 P015 10.1186/cc145 1 3 1998 1998 Current Science Ltd cc-2-s1-p015

Methods

The in vitro ability of G-CSF to improve neutrophil superoxide production has been previously demonstrated in ALF patients [1]. However, the in vivo effect on neutrophil function is unknown. Therefore G-CSF was given to three groups of ALF patients (n = 6) as a daily infusion at four different dosages (25, 50, 100 and 150 µg/m2). Superoxide production was measured after fMLP stimulation, before and at 24 and 96 h.

Results

See table

G-CSF significantly enhanced superoxide production at 96 h (P < 0.05). Furthermore, this effect was observed at doses below the standard therapeutic dose of 150 µg/m2. Further studies are needed to determine the therapeutic value of G-CSF in the prevention and treatment of infection in ALF patients.

<p>Table</p>

G-CSF

Hours after G-CSF infusion

µg/m2

0 h

24 h

96 h

25

221.4 (58.1)

267.9(62.7)

382.7 (40.6)

50

308.1 (17.2)

339.3 (56.3)

592.0 (98.7)

100

231.5 (65.2)

225.9 (52.6)

317.5(82.2)

150

217.4 (30.7)

357.0 (62.2)

358.4 (45.4)

<p>Effect of granulocyte colony stimulating factor (G-CSF) on neutrophil function in patients with acute liver failure.</p> Rolando N Clapperton M J Hepatol 1996 25 100