cc5794 CCJ Poster presentation <p>Effects of hypertonic saline solution and pentoxifylline on rat mesenteric microcirculation after hemorrhagic shock/reperfusion followed by cecal ligation/puncture: an intravital microscopic study</p> Nakagawa NK Nogueira RA Obuti CA Correia CJ Cruz JWMC de Figueiredo LF Poli e Silva M Rocha Sannomiya P

Research Division, Heart Institute (InCor), LIM-11 University of São Paulo Medical School, São Paulo – SP, Brazil

UNICID, São Paulo – SP, Brazil

Institute of Biomedical Sciences, University of São Paulo, Brazil

 Critical Care <p>Fourth International Symposium on Intensive Care and Emergency Medicine for Latin America</p> Meeting abstracts http://ccforum.com/supplements/notes/ccv11s3-info.pdf <p>Fourth International Symposium on Intensive Care and Emergency Medicine for Latin America</p> São Paulo, Brazil 20–23 June 2007 1364-8535 2007 11 Suppl 3 P7 http://ccforum.com/content/11/S3/P7 10.1186/cc5794 19 6 2007 2007 BioMed Central Ltd

Objective

Hemorrhagic shock/reperfusion followed by sepsis triggers systemic microcirculatory disturbances that may induce multiple organ failure. The present in-vivo study evaluated the effects of hypertonic saline solution (HSS) (7.5%, 4 ml/kg) and pentoxifylline (PTX) (4 mg/kg) on mesenteric microcirculation in double-injured rats.

Methods

Thirty-three anesthetized Wistar rats (~250 g) were randomly assigned to: (1) SHAM: no injury group; (2) HSS: hemorrhagic shock/reperfusion with HSS; (3) LR: hemorrhagic shock/reperfusion with lactated Ringer's solution (LR), three times shed blood volume; (4) HSS + PTX: hemorrhagic shock/reperfusion with HSS associated with PTX; and (5) LR + PTX: hemorrhagic shock/reperfusion with LR associated with PTX. The animals were submitted to cecal ligation/puncture (second injury).

Results

Leukocyte – endothelium interactions (Table 1) were assessed by intravital microscopy at mesenteric postcapillaric venules (~21.07 μm diameter).

<p>Table 1</p>

Leukocyte – endothelial interactions in mesenteric postcapillaric venules

Rollers

Adherent cells

Migrated cells

Total white blood

Group

n

(10 min)

(100 μm venule length)

(5,000 μm2)

(cells/mm3)

SHAM

6

96 ± 12

3 ± 1

2 ± 1

12,367 ± 2,641

LR

7

207 ± 19a

17 ± 1a

17 ± 1a

16,771 ± 5,703a

HSS

8

108 ± 16

9 ± 3c

12 ± 3c

12,388 ± 6,629

HSS + PTX

7

102 ± 26

6 ± 1b,c

6 ± 1b,c

10,757 ± 2,483

LR + PTX

5

115 ± 20

7 ± 1c

7 ± 1b,c

11,650 ± 1,570

aP < 0.001 vs other groups, cP < 0.001 vs SHAM, cP < 0.001 vs HSS.

Conclusion

The in-vivo observation of the rat mesenteric microcirculation showed that, in a double-injury model, reperfusion of the animals with HSS and PTX did attenuate the inflammation response compared with LR-reperfused animals.

Acknowledgements

Funded by FAPESP and PRONEX.