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Immunostaining of microvessels of guinea pig hearts with monoclonal antibody against heparan sulfate after minimal perfusion. Immunostaining of microvessels of guinea pig hearts with monoclonal antibody against heparan sulfate after minimal perfusion (panels A and B), after 15 minutes of ischemia followed b y 20 minutes of reperfusion in the absence (panels C and D) and presence of NO (panels G and H), and after the glycocalyx had been enzymatically degraded by means of heparinase (panels E and F). Specimens are paraffin-embedded sections of guinea pig heart. The original magnification was 20x (panels on the left hand side) and 100x (panels on the right hand side). VL = vascular lumen, HS = heparan sulfate.
Bruegger et al., Critical Care 2008, 12:R73 [View article]
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